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3 novel single nucleotide polymorphisms (SNPs) were being discovered as g.1782775A>G, g.1782924A>G and g.1782960T>C in the very first intron of LYZ gene. The partial DNA sequence of LYZ gene in donkeys was documented while in the existing study and sequences of LYZ have been entered to NCBI Genbank database Using the accession amount: MK984689-MK98